target selectorTM biomarkerS

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Biocept’s Target Selector™ platform features a range of liquid biopsy tests to assess NCCN guideline-driven-clinically actionable cancer biomarkers from a patient’s blood sample.

Increasing Access to Targeted Therapies via Liquid Biopsy
Test Technology Result Interpretation CPT Codes Method Targeted Therapies
ALK FISH Translocation 88377 CTC Xalkori®, Zykadia®, Alecensa®, Alunbrig®
AR Expression Expression 88346 or 88350 CTC Zytiga®, Xtandi®
BRAF Sequencing Mutation 81210 ctDNA Zelboraf®, Tafinlar®, Mekinist®
CTC Antibody
Capture
Enumeration 86152/86153 88346x1, 88350x2 CTC
EGFR
T790M,DEL19,L858R
Sequencing Mutation 81235 ctDNA Tarceva®, Gilotrif®, Iressa®, Tagrisso®
ER Expression Expression 88346 or 88350 CTC Nolvadex®, Aromatase Inhibitors
FGFR1 FISH Amplification 88377 CTC Nolvadex®, Faslodex®, Femara®, Arimidex®, Aromasin®
HER2 FISH Amplification 88377 CTC Herceptin®, Perjeta®, Tykerb®, Kadcyla®
KRAS Sequencing Mutation 81275 ctDNA Erbitux®, Vectibix®
MET FISH Amplification 88377 CTC Xalkori®, Cabometyx®, Exelixi®, Tivantinib®
NRAS Sequencing Mutation 81311 ctDNA Erbitux®, Vectibix®
PD-L1 Expression Expression 88346 or 88350 CTC Keytruda®, Opdivo®
PR Expression Expression 88346 or 88350 CTC Nolvadex®, Aromatase Inhibitors
RET FISH Translocation 88377 CTC Cabometyx®, Capresla®
ROS1 FISH Translocation 88377 CTC Xalkori®, Zykadia®, Alecensa®

ALK gene rearrangements are found in 2-7% of non-small cell lung cancer (NSCLC) cases, and detection is used to qualify patients for possible therapeutic intervention. In these rearrangements, the anaplastic lymphoma kinase (ALK) gene is fused to the echinoderm microtubule-associated protein-like 4 (EML4) gene. Multiple variants of this oncogenic EML4-ALK fusion have been reported; all involve the same cytoplasmic portion of the ALK protein, but with different truncations of EML4. Importantly, ALK rearrangements most commonly occur in the absence of EGFR or KRAS mutations.1

ALK gene rearrangements, or the resulting fusion proteins, may be detected in tumor specimens using fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), or reverse transcription polymerase chain reaction (RT-PCR).2 Biocept’s Target Selector™ liquid biopsy platform has the ability to identify ALK gene translocations in patients using a simple blood sample.

Detection of an ALK fusion is used to determine the likelihood of response to crizotinib (Xalkori®) or ceritinib (Zykadia®), two commercially available tyrosine kinase inhibitors.3 Additionally, alectinib (Alecensa®) and brigatinib (Alunbrig®) are approved for patients with ALK-positive metastatic NSCLC who have progressed on or are intolerant to crizotinib.

Targeted Therapies: Crizotinib (Xalkori®), ceritinib (Zykadia®), alectinib (Alecensa®), and brigatinib (Alunbrig®).

Methodology: Biocept’s ALK liquid biopsy test is performed on circulating tumor cells (CTCs), using FISH analysis to identify ALK gene rearrangements.

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References

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