Instructions                      
1. Choose Target Selector Assay from the drop-down menu                    
2. Enter number of reactions in yellow field. By default n is 37 reactions which includes 4 point standard curve in triplicates (12 reactions), 1 NTC and 24 test reactions. Choose n based on the number of reactions you intend to run.
3. By default Extra volume is set to  10%. If different, enter extra volume in blue field. This is used to calculate master mix.               
4. Wild Type: Volume of DNA template added to each well is 10µl . If test sample volumes are < 10 µl, make up the final volume to 10 µl using DNA suspension buffer      
5. EGFR Mutant : Volume of DNA template added to each well is 10 µl. If test sample volumes are < 10 µl, make up the final volume to 10 µl using DNA suspension buffer      
                       
Choose Target Selector Assay    
             
Fill in the number of reactions you intend to run (n)     Extra volume (%)   DNA Template (standard or test) volume(µl) 10    
                       
This table gives the pipetting volumes for EGFR Target Selector Assay setup                  
                       
Reagent Volume Volume per well (µl) Volume for n wells (µl)                  
                 
                 
Target Selector DNA Polymerase                  
Total  MasterMix (Excluding DNA template)                  
DNA Template (standard or test)                    
Total Reaction volume