This web page contains a live and functional spreadsheet created with
1. Choose Target Selector Assay from the drop-down menu
2. Enter number of reactions in yellow field. By default
is 37 reactions which includes 4 point standard curve in triplicates (12 reactions), 1 NTC and 24 test reactions. Choose
based on the number of reactions you intend to run.
3. By default Extra volume is set to 10%.
If different, enter extra volume in blue field. This is used to calculate master mix.
4. EGFR WT : Volume of DNA template is 3 ul. If test sample volumes are < 3 ul, make up the final volume to 3 ul using DNA suspension buffer
5. EGFR Mutant
: Volume of DNA template is 10 ul. If test sample volumes are < 10 ul, make up the final volume to 10 ul using DNA suspension buffer
Choose Target Selector Assay
Fill in the number of reactions you intend to run (n)
Extra volume (%)
DNA Template (standard or test) volume(
This table gives the pipetting volumes for EGFR Target Selector Assay setup
Volume per well (µl)
Volume for n wells (µl)
Target Selector DNA Polymerase
Total MasterMix (Excluding DNA template)
DNA Template (standard or test)
Total Reaction volume
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