Instructions                  
1. Choose Target Selector Assay from the drop-down menu                  
2. Enter number of reactions in yellow field. By default n is 37 reactions which includes 4 point standard curve in triplicates (12 reactions), 1 NTC and 24 test reactions. Choose n based on the number of reactions you intend to run.
3. By default Extra volume is set to  10%.  If different, enter extra volume in blue field. This is used to calculate master mix.             
4. EGFR WT : Volume of DNA template is 3 ul. If test sample volumes are < 3 ul, make up the final volume to 3 ul using DNA suspension buffer          
5. EGFR Mutant  : Volume of DNA template is 10 ul. If test sample volumes are < 10 ul, make up the final volume to 10 ul using DNA suspension buffer        
                   
Choose Target Selector Assay    
         
Fill in the number of reactions you intend to run (n)     Extra volume (%)   DNA Template (standard or test) volume(µl) 10
                   
This table gives the pipetting volumes for EGFR Target Selector Assay setup                  
                   
Reagent Volume Volume per well (µl) Volume for n wells (µl)              
             
Target Selector DNA Polymerase              
Total  MasterMix (Excluding DNA template)              
DNA Template (standard or test)                
Total Reaction volume