Instructions              
1. Choose Target Selector Assay from the drop-down menu              
2. By default n is 37 reactions which includes 4 point standard curve in triplicates (12 reactions), 1 NTC and 24 test reactions. Choose n based on the number of reactions you intend to run.
3. By default Extra volume is set to  10%. This is used to calculate master mix.             
4. EGFR WT: Volume of DNA template added to each well is 3µl . If test sample volumes are < 3 µl, make up the final volume to 3 µl using DNA suspension buffer    
5. EGFR Mutant : Volume of DNA template added to each well is 10 µl. If test sample volumes are < 10 µl, make up the final volume to 10 µl using DNA suspension buffer    
               
Choose Target Selector Assay
           
Fill in the number of reactions you intend to run (n) Extra volume (%)   DNA Template (standard or test) volume(µl)  
               
This table gives the pipetting volumes for EGFR Target Selector Assay setup          
Reagent Volume Volume per well (µl) Volume for n wells (µl)          
EGFR Assay premix          
Target Selector DNA Polymerase 0.8          
Total  MasterMix(Excluding DNA template)          
DNA Template (standard or test)            
Total Reaction volume 20